Figure S4 . " width="100%" height="100%">
Journal: Cell Reports Medicine
Article Title: The metabolic and cardiovascular effects of amphetamine are partially mediated by the central melanocortin system
doi: 10.1016/j.xcrm.2025.101936
Figure Lengend Snippet: AMPH acts through both the noradrenergic and serotonergic systems to impact the melanocortin system actions on metabolism and blood pressure (A) αMSH secretion from hypothalamic explants in response to artificial cerebrospinal fluid (aCSF), SB242084, CAR and PHE, aCSF + AMPH, SB242084 + AMPH, and CAR and PHE + AMPH. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, one-way ANOVA, Tukey’s multiple comparisons test. n = 6–21, mean ± SEM. (B) αMSH secretion from hypothalamic explants in response to aCSF, aCSF + AMPH, L-741626 + AMPH, SKF83566 + AMPH, and GR103691 + AMPH. ∗ p < 0.05, ∗∗ p < 0.01, ∗∗∗ p < 0.001, one-way ANOVA, Tukey’s multiple comparisons test. n = 6–7, mean ± SEM. (C) Percentage body weight change of DIO mice, 24 h post treatment with i.c.v. Veh + i.p. Veh; i.c.v. Veh + i.p. AMPH (5 mg/kg i.p.); i.c.v. SB242084 + i.p. Veh; i.c.v. SB242084 + i.p. AMPH (5 mg/kg); i.c.v. CAR and PHE + i.p. Veh; i.c.v. CAR and PHE + i.p. AMPH (5 mg/kg); i.c.v. CAR, PHE, and SB242084 + Veh; or i.c.v. CAR, PHE, and SB242084 + i.p. AMPH (5 mg/kg). One-way ANOVA, Tukey’s multiple comparisons test, ∗∗∗ p < 0.001, n = 5–8, mean ± SEM. (D) 24-h food intake (kcal) of DIO mice treated with i.c.v. Veh + i.p. Veh; i.c.v. Veh + i.p. AMPH (5 mg/kg); i.c.v. SB242084 + i.p. Veh; i.c.v. SB242084 + i.p. AMPH (5 mg/kg); i.c.v. CAR and PHE + i.p. Veh; i.c.v. CAR and PHE + i.p. AMPH (5 mg/kg); i.c.v. CAR, PHE, and SB242084 + Veh; or i.c.v. CAR, PHE, and SB242084 + AMPH (5 mg/kg). One-way ANOVA, Tukey’s multiple comparisons test, ∗ p < 0.05, ∗∗ p < 0.01, n = 5–8, mean ± SEM. (E) SBP (mmHg) in DIO mice treated with i.c.v. Veh and i.p. Veh or AMPH. n = 9, mean ± SEM. (F) Average SBP (mmHg) over 3 h post i.c.v. Veh and i.p. Veh or AMPH (5 mg/kg) treatment in DIO mice. ∗ p < 0.05, unpaired t test, n = 9, mean ± SEM. (G) SBP (mmHg) in DIO mice treated with i.c.v. Veh or SB242084 and i.p. Veh or AMPH (5 mg/kg). n = 6, mean ± SEM. (H) Average SBP (mmHg) over 3 h post i.c.v. Veh or SB242084 and i.p. Veh or AMPH (5 mg/kg) treatment in DIO mice. ∗ p < 0.05, unpaired t test, n = 6, mean ± SEM. (I) SBP (mmHg) in DIO mice treated with i.c.v. Veh or CAR, PHE and i.p. Veh or AMPH (5 mg/kg). n = 7–8, mean ± SEM. (J) Average SBP (mmHg) over 3 h post i.c.v. Veh or CAR, PHE and i.p. Veh or AMPH (5 mg/kg) treatment in DIO mice. Unpaired t test, n = 7–8, mean ± SEM. (K) SBP (mmHg) in DIO mice treated with i.c.v. Veh or CAR, PHE, SB242084 and i.p. Veh or AMPH (5 mg/kg). n = 4–6, mean ± SEM. (L) Average SBP (mmHg) over 3 h post i.c.v. Veh or CAR, PHE, SB242084 and i.p. Veh or AMPH (5 mg/kg) treatment in DIO mice. Unpaired t test, n = 4–6, mean ± SEM. (M) Heart rate (BPM) in DIO mice treated with i.c.v. Veh or CAR, PHE, SB242084 and i.p. Veh or melanotan II (MTII) (1 mg/kg i.p.). n = 4–6, mean ± SEM (all n are biological replicates). See also Figure S4 .
Article Snippet: After 1h equilibration period in aCSF at 37°, the brain slices were incubated for 45 min with different monoamine receptor antagonists, SB242084 (CAS 1049747-87-6 Tocris bioscience) (50nM), Carvedilol (CAR) (CAS 72956-09-3 Tocris bioscience) (50nM) and Phentolamine mesylate (PHE) (CAS 65-28-1 Tocris bioscience) (1μM), L-741626 (CAS 81226-60-0, Tocris bioscience) (50nM), SKF83566 hydrobromide (CAS 108179-91-5 Tocris bioscience) (10mM), GR103691 (CAS 162408-66-4 Tocris bioscience) (50nM) or aCSF (control) in aCSF.
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